Journal: bioRxiv

Article Title: Synthetic methanogenic communities reveal differential impact of ecological perturbations on aceto- and hydrogeno-trophic methanogens

doi: 10.1101/307041

Figure Lengend Snippet: (a) Schematic of possible interactions of the three species for converting lactate to methane. The three different species Desulfovibrio vulgaris ( Dv ), Methanococcus maripaludis ( Mm ) and Methanosarcena barkeri ( Mb ) are shown as blue, yellow, and red circles respectively. The metabolite concentrations shown are those based on the stoichiometries of reactions given in and using 30mM initial lactate. Possible thermodynamic inhibitions are indicated by t-ended arrows. The dashed line indicates possible co-utilization of H 2 by Mb . (b) Methane produced in the headspace in the absence of sulfate and in the different co- and tri-cultures as indicated on the x-axis. Measurements from 5 mL test tube cultures are used to extrapolate to 1 L culture output, so to achieve a better comparison of gas and organic acid data (in mM). (c, d) Lactate and acetate detected in the liquid phase after 21 days cultivation without sulfate addition. Red dots in these two panels refer to the three replicates in the Dv-Mb co-cultures. (replicate 1 – red hollow circle, replicate 2 – dashed red hollow circle, and replicate 3 – filled red circle). Error bars on panels b-d are based on three replicates.

Article Snippet: Desulfovibrio vulgaris Hildenborough (DSM644, Dv-WT), Methanosarcina barkeri (DSM800, Mb), and Methanococcus maripaludis S2 (DSM2067, Mm) were originally ordered from the public strain centre DSMZ ( www.dsmz.de ).

Techniques: Produced

Journal: bioRxiv

Article Title: Synthetic methanogenic communities reveal differential impact of ecological perturbations on aceto- and hydrogeno-trophic methanogens

doi: 10.1101/307041

Figure Lengend Snippet: Produced methane in the headspace after 21 days cultivation with 7.5 mM (a) and 15 mM (b) sulfate addition. The different co- and tri-cultures composed of species Desulfovibrio vulgaris ( Dv ), Methanococcus maripaludis ( Mm ) and Methanosarcena barkeri ( Mb ), as shown on the x-axis. Measurements from 5 mL test tube cultures are used to extrapolate to 1 L culture output, so to achieve a better comparison of gas and organic acid data (in mM; see also Fig. S2). Colors indicate different culturing periods as shown in the legend. Error bars on both panels are based on three replicates.

Article Snippet: Desulfovibrio vulgaris Hildenborough (DSM644, Dv-WT), Methanosarcina barkeri (DSM800, Mb), and Methanococcus maripaludis S2 (DSM2067, Mm) were originally ordered from the public strain centre DSMZ ( www.dsmz.de ).

Techniques: Produced

Journal: Cell reports

Article Title: Displacement of WDR5 from Chromatin by a WIN Site Inhibitor with Picomolar Affinity

doi: 10.1016/j.celrep.2019.02.047

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Molm13 , DSMZ , Cat# ACC554; RRID:CVCL_2119.

Techniques: Western Blot, Control, Recombinant, Protease Inhibitor, Reverse Transcription, Binding Assay, Proliferation Assay, Cloning, HMT Assay, Structural Proteomics, Plasmid Preparation, shRNA, Software